in situ hybridization rnascope multiplex fluorescent kit Search Results


94
ATCC rnascope multiplex fluorescent v2 assay acd
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Jackson Immuno donkey anti rabbit cy5
KEY RESOURCES TABLE
Donkey Anti Rabbit Cy5, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Advanced Cell Diagnostics Inc rnascope fluorescent multiplex (3-plex) reagent kit
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Rnascope Fluorescent Multiplex (3 Plex) Reagent Kit, supplied by Advanced Cell Diagnostics Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Advanced Cell Diagnostics Inc rnascope probes against rbfox3 (neun
A) Representative image demonstrating <t>RNAscope</t> visualization of frontal cortex deep layer neurons (NeuN, white), astrocytes (Glast, green), and <t>Bdnf</t> mRNA (red). DAPI, blue. Scale bar = 20 μm
Rnascope Probes Against Rbfox3 (Neun, supplied by Advanced Cell Diagnostics Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Akoya Biosciences tsa plus fluorescein detection kit akoya biosciences cat
A) Representative image demonstrating <t>RNAscope</t> visualization of frontal cortex deep layer neurons (NeuN, white), astrocytes (Glast, green), and <t>Bdnf</t> mRNA (red). DAPI, blue. Scale bar = 20 μm
Tsa Plus Fluorescein Detection Kit Akoya Biosciences Cat, supplied by Akoya Biosciences, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Akoya Biosciences tsa plus cyanine 5
A) Representative image demonstrating <t>RNAscope</t> visualization of frontal cortex deep layer neurons (NeuN, white), astrocytes (Glast, green), and <t>Bdnf</t> mRNA (red). DAPI, blue. Scale bar = 20 μm
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Bio-Techne corporation dapi
Fluorescent in situ hybridisation for ( a ) known coloboma gene targets and ( b ) OFC candidate genes identified in this <t>study</t> <t>counterstained</t> with <t>DAPI</t> nuclear stain on chick eyes at HH.St28, immediately prior to fusion. Note for EMX2 the OFM region highlights the POM region - expression was not observed in the NR or RPE. In both a and b , Top : grayscale RNAscope probe signal without DAPI highlighting mRNA signal in the immediate optic fissure margin region. Middle: mRNA probe and DAPI merged images of broad ventral eye region. Bottom: Dorsal eye region with probe and DAPI merged. OFM, optic fissure margin; NR, neural retina; POM, periocular mesenchyme; RPE, retinal pigmented epithelium. Scale = 50 μm.
Dapi, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ATCC chromium chip b single cell kit
Fluorescent in situ hybridisation for ( a ) known coloboma gene targets and ( b ) OFC candidate genes identified in this <t>study</t> <t>counterstained</t> with <t>DAPI</t> nuclear stain on chick eyes at HH.St28, immediately prior to fusion. Note for EMX2 the OFM region highlights the POM region - expression was not observed in the NR or RPE. In both a and b , Top : grayscale RNAscope probe signal without DAPI highlighting mRNA signal in the immediate optic fissure margin region. Middle: mRNA probe and DAPI merged images of broad ventral eye region. Bottom: Dorsal eye region with probe and DAPI merged. OFM, optic fissure margin; NR, neural retina; POM, periocular mesenchyme; RPE, retinal pigmented epithelium. Scale = 50 μm.
Chromium Chip B Single Cell Kit, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Bio-Techne corporation rnascope multiplex fluorescent reagent kit v2
Fluorescent in situ hybridisation for ( a ) known coloboma gene targets and ( b ) OFC candidate genes identified in this <t>study</t> <t>counterstained</t> with <t>DAPI</t> nuclear stain on chick eyes at HH.St28, immediately prior to fusion. Note for EMX2 the OFM region highlights the POM region - expression was not observed in the NR or RPE. In both a and b , Top : grayscale RNAscope probe signal without DAPI highlighting mRNA signal in the immediate optic fissure margin region. Middle: mRNA probe and DAPI merged images of broad ventral eye region. Bottom: Dorsal eye region with probe and DAPI merged. OFM, optic fissure margin; NR, neural retina; POM, periocular mesenchyme; RPE, retinal pigmented epithelium. Scale = 50 μm.
Rnascope Multiplex Fluorescent Reagent Kit V2, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Advanced Cell Diagnostics Inc rnascope multiplex fluorescent reagent kit v2 (520, 570, 650) assay
Fluorescent in situ hybridisation for ( a ) known coloboma gene targets and ( b ) OFC candidate genes identified in this <t>study</t> <t>counterstained</t> with <t>DAPI</t> nuclear stain on chick eyes at HH.St28, immediately prior to fusion. Note for EMX2 the OFM region highlights the POM region - expression was not observed in the NR or RPE. In both a and b , Top : grayscale RNAscope probe signal without DAPI highlighting mRNA signal in the immediate optic fissure margin region. Middle: mRNA probe and DAPI merged images of broad ventral eye region. Bottom: Dorsal eye region with probe and DAPI merged. OFM, optic fissure margin; NR, neural retina; POM, periocular mesenchyme; RPE, retinal pigmented epithelium. Scale = 50 μm.
Rnascope Multiplex Fluorescent Reagent Kit V2 (520, 570, 650) Assay, supplied by Advanced Cell Diagnostics Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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96
fluidigm c1 gene expression omnibus repository
Fluorescent in situ hybridisation for ( a ) known coloboma gene targets and ( b ) OFC candidate genes identified in this <t>study</t> <t>counterstained</t> with <t>DAPI</t> nuclear stain on chick eyes at HH.St28, immediately prior to fusion. Note for EMX2 the OFM region highlights the POM region - expression was not observed in the NR or RPE. In both a and b , Top : grayscale RNAscope probe signal without DAPI highlighting mRNA signal in the immediate optic fissure margin region. Middle: mRNA probe and DAPI merged images of broad ventral eye region. Bottom: Dorsal eye region with probe and DAPI merged. OFM, optic fissure margin; NR, neural retina; POM, periocular mesenchyme; RPE, retinal pigmented epithelium. Scale = 50 μm.
C1 Gene Expression Omnibus Repository, supplied by fluidigm, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


KEY RESOURCES TABLE

Journal: Cell reports

Article Title: Systematic analysis of purified astrocytes after SCI unveils Zeb2os function during astrogliosis

doi: 10.1016/j.celrep.2021.108721

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Donkey anti-rabbit Cy5 , Jackson ImmunoResearch Labs , Cat# 711–175-152; RRID: AB_2340607.

Techniques: Virus, shRNA, Plasmid Preparation, Purification, Recombinant, Multiplex Assay, Transfection, RNAscope, Negative Control, Sequencing, Software, Flow Cytometry, Fluorescence, Microscopy, Laser-Scanning Microscopy

A) Representative image demonstrating RNAscope visualization of frontal cortex deep layer neurons (NeuN, white), astrocytes (Glast, green), and Bdnf mRNA (red). DAPI, blue. Scale bar = 20 μm

Journal: Neuron

Article Title: Loss of adaptive myelination contributes to methotrexate chemotherapy-related cognitive impairment

doi: 10.1016/j.neuron.2019.04.032

Figure Lengend Snippet: A) Representative image demonstrating RNAscope visualization of frontal cortex deep layer neurons (NeuN, white), astrocytes (Glast, green), and Bdnf mRNA (red). DAPI, blue. Scale bar = 20 μm

Article Snippet: RNAscope was performed according to Fluorescent Multiplex Reagent Kit protocol using RNAscope probes against BDNF (Advanced Cell Diagnostics, 424821), RbFox3 (NeuN; Advanced Cell Diagnostics 313311-C2) and Slc1a3 (Glast; Advanced Cell Diagnostics, 320851-C3).

Techniques:

Fluorescent in situ hybridisation for ( a ) known coloboma gene targets and ( b ) OFC candidate genes identified in this study counterstained with DAPI nuclear stain on chick eyes at HH.St28, immediately prior to fusion. Note for EMX2 the OFM region highlights the POM region - expression was not observed in the NR or RPE. In both a and b , Top : grayscale RNAscope probe signal without DAPI highlighting mRNA signal in the immediate optic fissure margin region. Middle: mRNA probe and DAPI merged images of broad ventral eye region. Bottom: Dorsal eye region with probe and DAPI merged. OFM, optic fissure margin; NR, neural retina; POM, periocular mesenchyme; RPE, retinal pigmented epithelium. Scale = 50 μm.

Journal: bioRxiv

Article Title: Identification of novel coloboma candidate genes through conserved gene expression analyses across four vertebrate species

doi: 10.1101/2022.12.22.521600

Figure Lengend Snippet: Fluorescent in situ hybridisation for ( a ) known coloboma gene targets and ( b ) OFC candidate genes identified in this study counterstained with DAPI nuclear stain on chick eyes at HH.St28, immediately prior to fusion. Note for EMX2 the OFM region highlights the POM region - expression was not observed in the NR or RPE. In both a and b , Top : grayscale RNAscope probe signal without DAPI highlighting mRNA signal in the immediate optic fissure margin region. Middle: mRNA probe and DAPI merged images of broad ventral eye region. Bottom: Dorsal eye region with probe and DAPI merged. OFM, optic fissure margin; NR, neural retina; POM, periocular mesenchyme; RPE, retinal pigmented epithelium. Scale = 50 μm.

Article Snippet: Slides were then mounted in Fluorsave (Merck #345789) with and counterstained with DAPI (ACD Bio-techne, RNAscope Multiplex Fluorescent Reagent Kit v2 #323100).

Techniques: In Situ, Hybridization, Staining, Expressing