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Image Search Results
Journal: Cell reports
Article Title: Systematic analysis of purified astrocytes after SCI unveils Zeb2os function during astrogliosis
doi: 10.1016/j.celrep.2021.108721
Figure Lengend Snippet: KEY RESOURCES TABLE
Article Snippet:
Techniques: Virus, shRNA, Plasmid Preparation, Purification, Recombinant, Multiplex Assay, Transfection, RNAscope, Negative Control, Sequencing, Software, Flow Cytometry, Fluorescence, Microscopy, Laser-Scanning Microscopy
Journal: Neuron
Article Title: Loss of adaptive myelination contributes to methotrexate chemotherapy-related cognitive impairment
doi: 10.1016/j.neuron.2019.04.032
Figure Lengend Snippet: A) Representative image demonstrating RNAscope visualization of frontal cortex deep layer neurons (NeuN, white), astrocytes (Glast, green), and Bdnf mRNA (red). DAPI, blue. Scale bar = 20 μm
Article Snippet: RNAscope was performed according to Fluorescent Multiplex Reagent Kit protocol using
Techniques:
Journal: bioRxiv
Article Title: Identification of novel coloboma candidate genes through conserved gene expression analyses across four vertebrate species
doi: 10.1101/2022.12.22.521600
Figure Lengend Snippet: Fluorescent in situ hybridisation for ( a ) known coloboma gene targets and ( b ) OFC candidate genes identified in this study counterstained with DAPI nuclear stain on chick eyes at HH.St28, immediately prior to fusion. Note for EMX2 the OFM region highlights the POM region - expression was not observed in the NR or RPE. In both a and b , Top : grayscale RNAscope probe signal without DAPI highlighting mRNA signal in the immediate optic fissure margin region. Middle: mRNA probe and DAPI merged images of broad ventral eye region. Bottom: Dorsal eye region with probe and DAPI merged. OFM, optic fissure margin; NR, neural retina; POM, periocular mesenchyme; RPE, retinal pigmented epithelium. Scale = 50 μm.
Article Snippet: Slides were then mounted in Fluorsave (Merck #345789) with and counterstained with
Techniques: In Situ, Hybridization, Staining, Expressing